The analysis used the 16S V4 region, 97 percent OTU clustering and rarefaction to 1,150 reads per sample, retaining 1,050 OTUs. Samples were processed across sequencing laboratories, with eight rerun. No aged-whiskey sensory panel was conducted; community profiles cannot establish tasting outcomes.
Methods: DNA extraction, sequencing and sequence processing; Discussion
Methods: DNA extraction, sequencing and sequence processing; Discussion
Reviewed main HTML and seven figures; section locator checked. Supplement unavailable; source remains Partial Source.
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Evidence
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The analysis used the 16S V4 region, 97 percent OTU clustering and rarefaction to 1,150 reads per sample, retaining 1,050 OTUs. Samples were processed across sequencing laboratories, with eight rerun. No aged-whiskey sensory panel was conducted; community profiles cannot establish tasting outcomes.
Location and context
Methods: DNA extraction, sequencing and sequence processing; Discussion. Main article HTML reviewed; original section/table/figure labels used instead of unverified PDF page locators.
Annotation
Verification concerns fidelity to the reviewed main article, not independent validation of causality. Supplement remains unavailable; no supplement-only findings asserted.
Verification checklist
Liu 2023 BC8 — Sequencing design limits downstream flavor inference
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